The individuals were arrested at Agogo in the Asanti Akim North District of the Ashanti Region.
The teenager is alleged to have stolen from an agrochemical shop owned by one of the suspects in Agogo Market.
One of the suspects in a video recording inserted a stick into the anus of the boy while the others cheer him on.
Konongo Divisional Commander Chief Superintendent Shibu Abubakar Osei said that they are on a manhunt for other suspects in the case.
"Police had information that was on the 9th of July 2021, both hands of the boy was tied behind him and he was on the ground with his face down. His trousers were removed halfway and I overheard the guys around telling the elderly man to insert it and the elderly man also inserted the stick..."
The teenage boy is currently receiving treatment at the Agogo Presbyterian Hospital.
The Medical Superintendent of the hospital Dr. William Nana said he will be sent to the theatre tomorrow for further examination.
"For now he is stable just that he has a fever so we have given him antibiotics. Tomorrow he will be sent to the theatre and we will see if the rectum has been perforated. The pain is so much that he won't even allow you to examine the anus."